bioluminescent plants .... real... have your own mini pandora of glowing plants

[arkfussion]

Hello, I had recently became interested with the concept of glowing organisms and have since transfected bioluminescent plasmid dna (Pvib) into ecoli cells to produce a blue glowing strain... glowing plants are not a far jump from this point. I was wondering the maximum you would be willing to purchase a blue glowing plant.. just because im wondering if it would be economic to follow through with this. and might sell them. pandora plants : )

[visualizer]

Well, first I would want undoctored photo proof. All "bioluminescent" plants that have been genetically engineered to have VISIBLE light coming from them have all used phosphorescent plasmids, not true bioluminescent ones (to my knowledge). So, I would need clarification of that as well. I know of the Pvib plasmid. However, to date, I have found to research papers documenting successful transfection of this plasmid into plants and having it work without chemical or bacterial additives. Can you explain in detail how you activated the Pvib plasmid, perhaps?

But more to the point, yes I would be VERY interested. And I would be willing to pay in the $100-200 range if the plants could successfully be bred as well. If not, I'd go for the $30 range or so. Also, a variety of plant species is a must! :D

[stubydub]

id love that id pay 40 maby 50 if i was in a good mood that would be awsome:)

[CiaphasCain]

If it's bright enough to light up a 'paper screen' at night, then I'm all for em! (Yay night time lighting without electricity consumption!)

[arkfussion]

Well, first I would want undoctored photo proof. All "bioluminescent" plants that have been genetically engineered to have VISIBLE light coming from them have all used phosphorescent plasmids, not true bioluminescent ones (to my knowledge). So, I would need clarification of that as well. I know of the Pvib plasmid. However, to date, I have found to research papers documenting successful transfection of this plasmid into plants and having it work without chemical or bacterial additives. Can you explain in detail how you activated the Pvib plasmid, perhaps?

But more to the point, yes I would be VERY interested. And I would be willing to pay in the $100-200 range if the plants could successfully be bred as well. If not, I'd go for the $30 range or so. Also, a variety of plant species is a must! :D

There are biolunimescent mustard and tabaco plants, or at least were.
The pvib plasmid contains all of the necessary lux genes to code for complete bio luminescence, no additional substrate is required ... also im planing on using a gaussia based plasmid due to the 1000 fold light output increase and lower cell energy consumption. Electorate a plasmolisized cell culture and grow single cells into functional a-sexual reproducing plants.

[visualizer]

There are biolunimescent mustard and tabaco plants, or at least were. It's my understanding that the pictures taken of these plants was only possible with special equipment. They weren't taken to represent what someone could actually see. The light they produced was too dim.

Regardless, I hope you can achieve what you've set forth to do. I'm looking forward to it.

And if you don't mind me asking, what technique did you use to transfect the plasmid? And are you working in a research lab or is this a DIY Bio project?

[arkfussion]

It's my understanding that the pictures taken of these plants was only possible with special equipment. They weren't taken to represent what someone could actually see. The light they produced was too dim.

Regardless, I hope you can achieve what you've set forth to do. I'm looking forward to it.

And if you don't mind me asking, what technique did you use to transfect the plasmid? And are you working in a research lab or is this a DIY Bio project?

correct, those have used a light sensitive film to capture the image of the tabacco plant. but that wont stop me from trying, my transfected ecoli are quite bright.
although i work at LANSCE, at the neutron scattering center (lujan center) and we have a rather large bio lab i hardly ever make use of it and stick mainly to a DIY technique. I use a homemade rotary-pulsed 8 kilo-volt electroporator which works pretty darn good.
-This might make a good science fair project now that i think of it-

[tm20]

well, asking how much people would pay for them you're maybe going to get answers like "5 cents" because people are people... they want to pay as least as possible :rotfl: i would be willing to pay a decent amount for them. ofcourse, this depends on how big the plant is but over here, small pot plants are around $3. i never go to the nursery so i wouldn't know too much about proces, but other than people buying them because they're pretty bioluminescence has many potentials such as:
-lighting up streets (would be probably unlikely that they can give off as much light as street lights we have now, but really, why do we have so many street lights when we already have headlights on our cars?! -_-)
-indicators for plants when they need water (so when its dry it glows, this can save lots of water)

there were a few more i read about, i just forgot... but anyway, good luck with your e coli bacteria :nsmile:

[visualizer]

so if this is mostly DIY, WHERE did you get the plasmid lux? I can't find any suppliers :( I was thinking of getting into DIY Bio, but the lack of suppliers, equipment, and money is really an issue atm...

[Neytiri te Chaka Mo'at'it]

yeah, i would be willing to pay upwards of 50 USD, however most people would not be willing to pay more than a typical plant